Monday, February 20, 2006
Everything that could go wrong went wrong in yesterday's pharmacology lab session. And I thought Miss V was exaggerating when she cautioned us last week about problems that could occur during the experiment.
Our aim was to measure the effect of histamine on the guinea pig ileum; Darren, Di and I were in charge of one organ bath. And boy.. wutta looonnngggg frustrating day! We were already in the lab by 1.30pm. Started our series of dilutions of the histamine stock solution, and everything seemed to be just fine at first.
But.. hey, what's this?! A kink in the drainage hose? Ok.. fine. Let's just hope that the drug can be properly drained from the organ bath during washing. Then.. do.. do.. do.. Oh! What's this??!! We got a tissue that behaves like a nutter? Man, our ileum was contracting like crazy. Talk about being hyperactive.
The two problems combined, the three of us were on our way to disaster and inevitable failure. By then, understandably (correct spelling ka?), Di and Darren started cursing left and right. The other groups were all smooth sailing, (well, except for one or two other groups that somehow managed to kill off their tissue by drug overdose, haha!) and we were in trouble, but then Miss V, seemed more content to help other teams at the back. For the sake of keeping our sanity, I tried to calm the 2 of them down and talked as gently as I could, eventhough my patience too was really tested. We were so frustrated, see. We were already in the lab for 2 solid hours, and not making any progress.
In the end, after another repitition of serial dilutions and after changing to another organ bath and ileum tissue, I made a brief sign of the cross, and we made a third attempt to record the tissue's contraction... and.. finally.. everything went on perfectly! Ok, I may sound like a total nerd here, but the ileum behaved really well and was so stable that I could just cry out in relief. Thank God. The lab session is really an important one for us, because it will contribute 30% to our final mark for pharmacology module.
Needless to say, we were the last group to finish. At 6.10pm lagi tu. Sigh... Dont mind me, I'm just blowing off some steam.